Reducing Retinal Blindness Worldwide

James Monaghan, PhD



Biology Department

Northeastern University

Boston, Massachusetts

 

BASIC RESEARCH PROJECT

Stem cell fate determination during axolotl retina regeneration

Scientific Summary: Degeneration of the human retina is irreversible, but Mexican axolotl salamanders have an amazing ability to regenerate their retinas even after their complete removal. We  aim to understand the cellular and molecular mechanisms that enable salamander retinal regeneration, with the goal of eventually applying this knowledge to restoring damaged retinas in humans.

Plans in 2023

This project aims to understand the molecular mechanisms that drive axolotl regeneration of retinal cells.

Throughout 2023, Dr. Monaghan’s laboratory will perform imaging of live, genetically modified axolotls that allows the visualization of cell behavior in the regenerating retina. This will be used to identify the cell sources that contribute to the regenerating retina. His team will also compare two different injury models to determine if different cells that mediate regeneration arise from different regions of the eye. Further, they will also compare the molecular signaling between the two injury models to determine if they use common cell signaling to regenerate the retina.

Specific Aims:

Aim 1 will perform in-vivo imaging of regenerating retinas in the axolotl.

Aim 2 will test whether specific cell signaling pathways identified in our 2022 funding cycle are required for retina regeneration.

Progress in 2022

Analysis of Notch Signaling-mediated Cell Fate determination during Regeneration of the Neural Retina

Dr. Monaghan’s findings from the 2022 funding period finalize the histological description of retinal regeneration, completed the first transcriptomic analysis and multiplexed imaging of gene expression, and confirmed that the Notch signaling pathway plays a critical role in retinal regeneration. This work has culminated in a manuscript that has undergone one round of review and revision and should be accepted in the upcoming days. Over the past two years, Dr. Monaghan has also begun to identify the cell types activated and the molecular mechanisms driving the overall response.

Dr. Monaghan proposed to refine his understanding of the role of Notch signaling in retinal regeneration.  His laboratory team characterized the activity of specific components of this signaling cascade as a new retina forms and give rise to photoreceptors neurons and other cell types.

Specific Aims: The lab investigated how Notch effector genes Hes1 and Hes5 control regeneration of specific cell types in a regenerating retina, focusing on cells of the regenerative retinal pigment epithelial layer. They also engineered a line of transgenic axolotls that will fluorescently reveal Notch activity in specific cells, allowing greater understanding of the mechanisms by which Notch signaling controls retinal regeneration.

Progress in 2021

Dr. Monaghan’s findings from the 2021 funding period have confirmed that the Notch signaling pathway plays a critical role in retinal regeneration. His laboratory team has shown that Notch controls regeneration of photoreceptor neurons and that two of its effector genes, Hes1 and Hes5, are expressed differentially in a regenerating retina. They have also shown that the axolotl retina likely regenerates from the cells of the retinal pigmented epithelium, identifying for the first time the retinal stem cell population in this animal model.

Progress in 2020

In 2020, Dr. Monaghan developed a technique that reveals cell types in the axolotl retina and their gene activity, which will equip the research team to understand the molecular mechanisms behind regeneration.  The team collected preliminary data suggesting that the Notch signaling pathway may dictate what types of cells regrow in the new retina.  Dr. Monaghan’s research confirmed that the regenerated retina re-establishes its connection with the brain, which is necessary for its functionality.  The research also showed for the first time that the axolotl retina contains a type of glial cell that may serve as stem cells during salamander retinal regeneration.

Results Previously Published: In eyes of newt and axolotl salamanders, the retinal pigment epithelium layer, which is adjacent to the neural retina, provides progenitor cells that rebuild the retina after an injury (Islam et al., 2014; Svistunov and Mitashov 1983). Notch signaling pathway, which facilitates inter-cellular communication and regulates gene expression, is active in the retinal pigment epithelium of the regenerating salamander eye, and its perturbation accelerates regeneration of retinal neurons (Nakamura and Chiba 2007). Notch signaling is also active in the mouse retinal pigment epithelium and can induce cultured mouse retinal cells to adopt some features of retinal photoreceptor neurons (Ha et al., 2017; Osakada et al., 2007). These results suggest that Notch signaling and its target genes may provide the key to unlocking regenerative potential in human retinas.

Print Friendly, PDF & Email


Change Font Size

Mission of RRF

The mission of the Retina Research Foundation is to reduce retinal blindness worldwide by funding programs in research and education. As a public charity, RRF raises funds from the private sector and the investment of its endowment funds.